Onza's patent pending technology for trapping gas has proven effective by third-party testing, not only trapping gas, but extending the useful life of the trapped gas.
Onza has invented a novel product for enhanced food safety that will disrupt the market. No competing product exists, because we have accomplished something that has never been done before. With the invention of Ozonate, Onza has unlocked the beneficial properties of ozone allowing it to be used whenever and wherever you want, in a solid, controllable and stable form. Ozonate has the potential to replace current sterilization methods, and make use of technology like MAP (Modified Atmosphere Packaging) unnecessary or simply a support system for our product.
At Stanford University the first testing of Ozonate was carried out to assess its effectiveness in preserving and extending the shelf-life of food products.
A solution of E. Coli was prepared and then deposited onto the leaves of spinach. These leaves were placed into separate beakers with glass test tube filled with test compounds, and placed into a 37°C incubator. Samples were assessed visually daily and given a quality rating (0-5).
The Ozonate treated spinach was able to maintain a perfect score of 5 an astounding 3 days in this hostile environment, whereas all other spinach samples were completely disintegrated.
Testing carried out by a third-party ISO-17025 accredited food laboratory used bulk spinach obtained from a national organic retailer to inoculate with E. Coli for testing. Spinach was sealed in separate containers with dual controls and Ozonate with and without solubilization. Bags were incubated for 24 hours and then samples were plated to determine the concentration of E. Coli present (cfu/gram). Ozonate was able to decrease total plate count as well as E. Coli to less than 1% of the spiked control (>99% kill). This study was performed in duplicate.
The same test performed with spinach was repeated, but in this test the spinach was spiked with Staphylococcus Aureus, Pseudomonas Aeruginosa, and pathogenic E. Coli O157:H7 strains. After incubation for 24 hours, these organisms were plated to measure the effects of Ozonate on the total populations. Ozonate was able to decrease Staphylococcus Aureus by 92.5%, Pseudomonas Aeruginosa by 96% and pathogenic E. Coli 157:H7 by 63%. Testing was conducted by a third-party ISO-17025 accredited food laboratory
At Stanford University the effectiveness of Ozonate as a gaseous sanitizing agent was assessed via an Minimum Inhibitory Concentration (MIC) assay.
A master solution of E. Coli with an effective OD of 0.001 was prepared, then 200 µL was serial diluted over 11 wells in triplicate (maximum concentration of ~8 x 107 cells). In separate rows above, 3g of Ozonate was distributed evenly among the wells. Risers added to each of the corner wells, the plate cover placed on top, and everything sealed air-tight. Plates were added into a 37°C incubator for 24 hours before their growth rates were assessed via a plate spectrometer.
Ozonate was able to consistently ablate 95% of all E. Coli, including from the maximum concentration wells which have an effective concentration that is 32X greater than standard MIC concentrations.
At a third-party ISO-17025 accredited laboratory, the same test performed with spinach was repeated exactly but with Ozonate that had been aged for 21 days. Again, Ozonate was able to kill over 99% of E. Coli spiked onto the spinach showing effectiveness after long storage periods.
In a test performed in Onza’s lab over 21 days, raspberries were kept in sealed bags at room temperature. At the completion of the test the control samples that contained only raspberries with no added Ozonate clearly showed advanced mold growth, however, the sample that had Onza’a Ozonate material in the bag showed no signs of mold at all.
At Stanford University the same Minimum Inhibitory Concentration (MIC) assay was performed as was performed and showed effective levels of ablation 57 days after the ozone was trapped to form Ozonate! Historically ozone only lasts for between 30min to four hours depending on the environment, Onza is still using effective amounts of Ozonate nearly two months after it was produced!
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